The polyester polyurethanase gene (pueA) fromPseudomonas chlororaphisencodes a lipase
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چکیده
منابع مشابه
The polyester polyurethanase gene (pueA) from Pseudomonas chlororaphis encodes a lipase.
A gene (pueA, polyurethane esterase A) encoding an extracellular polyurethanase (PueA) was cloned from Pseudomonas chlororaphis into Escherichia coli. The enzyme secreted from E. coli showed esterase activity when assayed with p-nitrophenyl acetate. Subcloning of a 3. 2-kb SalI-EcoRI fragment into a T7 RNA polymerase expression vector (pT7-6) produced a (35)S-labeled protein of 65 kDa. Nucleoti...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2000
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2000.tb09056.x